Holyrood is a city in Ellsworth County, Kansas, United States. As of the 2010 census, the city population was 447.
Holyrood is located at 38°35′15″N 98°24′43″W / 38.5875°N 98.41194°W / 38.5875; -98.41194 (38.587543, -98.412050). According to the United States Census Bureau, the city has a total area of 0.4 square miles (1.0 km²), all land.
As of the census of 2000, there were 464 people, 213 households, and 138 families residing in the city. The population density was 1,238.4 people per square mile (484.2/km²). There were 265 housing units at an average density of 707.3 per square mile (276.5/km²). The racial makeup of the city was 98.49% White, 1.08% from other races, and 0.43% from two or more races. Hispanic or Latino of any race were 2.16% of the population.