Bentleyville is a borough in Washington County, Pennsylvania, United States. The population was 2,502 at the 2000 census.
Bentleyville is located at 40°7′11″N 80°0′22″W / 40.11972°N 80.00611°W / 40.11972; -80.00611 (40.119799, -80.006146). U.S. Interstate 70 borders the borough's northern side and Pennsylvania state Route 917 forms Main Street.
According to the United States Census Bureau, the borough has a total area of 3.7 square miles (9.6 km²), all land; except Pigeon Creek passes through the borough.