Jonesville is a town in and the county seat of Lee County, Virginia, United States. The population was 995 at the 2000 census.
Jonesville was established in 1794 as the county seat of Lee County. The town was known as Glade Spring in its infancy. It was incorporated in 1884, and re-incorporated in 1901. It is named for Frederick Jones, a landowner.
Jonesville was a small but thriving center of local commerce in the late nineteenth and early twentieth centuries, but like most towns in the central Appalachian region, it fell into gradual economic decline as the coal boom ebbed in the latter half of the twentieth century. By the end of the century much of the commercial real estate in its downtown area was either unoccupied or underutilized. Most of Jonesville's remaining commercial activity is concentrated in the west end. Jonesville Drug, one of the oldest surviving downtown businesses, relocated to the old Chappell's Dairy site on the west end in 2004.