Sistersville is a city in Tyler County, West Virginia, along the Ohio River. The population was 1,588 at the 2000 census. The Sistersville Ferry crosses the Ohio River to the unincorporated community of Fly in Monroe County, Ohio.
Sistersville is located at 39°33′42″N 80°59′52″W / 39.56167°N 80.99778°W / 39.56167; -80.99778 (39.561615, -80.997791).
According to the United States Census Bureau, the city has a total area of 0.5 square miles (1.3 km²), all of it land.