Chiefland is a city in Levy County, Florida, United States. The population was 1,993 in the 2000 census. and was estimated at 2,095 in 2005. Chiefland calls itself the "Gem of the Suwannee Valley" and was incorporated in 1929.
A village of the Timucua was once located south of the present city and at Manatee Springs. The area's economy was traditionally based on agriculture, primarily farming (corn, watermelons, hay); ranching (cattle, hogs); dairy (milk); timber (pulpwood, lumber, turpentine) and aquaculture (fishing, oystering, crabbing).
Manatee Springs State Park is located six miles (10 km) west of town and the crystal-clear water is a first magnitude spring that flows directly into the Suwannee River. The park offers a full slate of activities, including camping. Manatees can be seen in the spring year-round, but especially in late Fall and Winter, where the constant 72°F (23°C) temperature of the spring is much warmer than river water.