Mayo is a town and the only municipality in Lafayette County, Florida, United States. The population was 988 at the 2000 census. As of 2004, the population recorded by the U.S. Census Bureau is 1,009 . It is the county seat of Lafayette County.
Mayo is located at 30°03′07″N 83°10′32″W / 30.051947°N 83.175676°W / 30.051947; -83.175676.
According to the United States Census Bureau, the town has a total area of 2.1 km² (0.8 sq mi), all land.