Macksville is a city in Stafford County, Kansas, United States. As of the 2010 census, the city population was 549.
According to the United States Census Bureau, the city has a total area of 1.0 square miles (2.6 km²), all of it land.
As of the census of 2000, there were 514 people, 191 households, and 127 families residing in the city. The population density was 513.1 people per square mile (198.5/km²). There were 227 housing units at an average density of 226.6 per square mile (87.6/km²). The racial makeup of the city was 81.71% White, 1.36% Native American, 0.19% Asian, 14.59% from other races, and 2.14% from two or more races. Hispanic or Latino of any race were 23.54% of the population.