Bernice is a town in Union Parish, Louisiana, United States. The population was 1,809 at the 2000 census. It is part of the Monroe Metropolitan Statistical Area.
The area was part of the Louisiana frontier and pine forests, but early houses survive from the mid-nineteenth century: among the oldest homes are the R.T. Moore/Gresham/Stenzel House, known as the "Sweet Onion", and McCuller Log Cabin, both circa 1865.
Bernice was established in 1899 as a sawmill town after Captain C.C. Henderson built the Arkansas Southern Railroad, the first railway in Union Parish. Also an agricultural trade center, Bernice is the youngest European-American town to be founded in the parish. The area was known as the "big woods" because of its large stands of huge virgin pine. Henderson built the railroad to enable harvesting of lumber from the area. Moving south from Junction City, he directed construction of the railroad to Winnfield.