Highlandville is a city in Christian County, Missouri, United States. The population was 872 at the 2000 census. It is part of the Springfield, Missouri Metropolitan Statistical Area.
Highlandville is located at 36°56′34″N 93°17′11″W / 36.94278°N 93.28639°W / 36.94278; -93.28639 (36.942690, -93.286447).
According to the United States Census Bureau, the city has a total area of 5.0 square miles (12.9 km²), of which, 5.0 square miles (12.9 km²) of it is land and 0.20% is water.