Humansville is a city in Polk County, Missouri, United States. The population was 946 at the 2000 census. It is part of the Springfield, Missouri Metropolitan Statistical Area.
Humansville is located at 37°47′44″N 93°34′42″W / 37.79556°N 93.57833°W / 37.79556; -93.57833 (37.795645, -93.578354). It sits on Brush Creek and the line of the old Kansas City, Clinton, and Southern Railway. It is 18 miles northwest of Bolivar, the seat of Polk County.
According to the United States Census Bureau, the city has a total area of 1.2 square miles (3.1 km²), all of it land. As of the 2000 census there were 946 people living in the city.