Keytesville is a town in Chariton County, Missouri, United States. The population was 533 at the 2000 census. It is the county seat of Chariton County.
Keytesville is located on the Lewis and Clark Trail, now U.S. Route 24. It is 10 miles east of Brunswick and 30 miles west of Moberly. Keytesville is located at 39°26′3″N 92°56′13″W / 39.43417°N 92.93694°W / 39.43417; -92.93694 (39.434144, -92.936967). According to the United States Census Bureau, the city has a total area of 0.7 square miles (1.8 km²), all of it land.
As of the census of 2000, there were 533 people, 253 households, and 129 families residing in the city. The population density was 764.8 people per square mile (294.0/km²). There were 295 housing units at an average density of 423.3 per square mile (162.7/km²). The racial makeup of the city was 95.31% White, 3.94% African American, 0.19% from other races, and 0.56% from two or more races. Hispanic or Latino of any race were 0.19% of the population.