Koshkonong is a city in Oregon County, Missouri, United States. The population was 205 at the 2000 census.
Koshkonong was once part of southern Missouri's fruitbelt that sprung up along the railroads in the area. The orchards have since been replaced with pasture land.
Koshkonong is located at 36°35′43″N 91°38′43″W / 36.59528°N 91.64528°W / 36.59528; -91.64528 (36.595356, -91.645360).