Urbana is a city in Dallas County, Missouri, United States. The population was 407 at the 2000 census. It is part of the Springfield, Missouri Metropolitan Statistical Area.
Urbana is located at 37°50′38″N 93°9′59″W / 37.84389°N 93.16639°W / 37.84389; -93.16639 (37.844025, -93.166316).
According to the United States Census Bureau, the city has a total area of 0.9 square miles (2.5 km²), all of it land.