Mathiston is a town in Choctaw and Webster Counties in the U.S. state of Mississippi. The population was 720 at the 2000 census.
Mathiston is located at 33°32′12″N 89°7′36″W / 33.53667°N 89.12667°W / 33.53667; -89.12667 (33.536549, -89.126529). Most of the town is in Webster County with a portion on the south side extending into Choctaw County. In the 2000 census, 643 of the town's 720 residents (89.3%) lived in Webster County and 77 (10.7%) in Choctaw County.
According to the United States Census Bureau, the town has a total area of 2.5 square miles (6.4 km²).None of the area is covered with water.