Holstein is a village in Adams County, Nebraska, United States. The population was 229 at the 2000 census. It is part of the Hastings, Nebraska Micropolitan Statistical Area.
Holstein is located at 40°27′53″N 98°39′0″W / 40.46472°N 98.65°W / 40.46472; -98.65 (40.464769, -98.649877).
According to the United States Census Bureau, the village has a total area of 0.2 square miles (0.6 km²), all of it land.