Millersburg is a village in Holmes County, Ohio, in the United States. As of the 2000 census, the village population was 3,326. It is the county seat of Holmes County.
Millersburg is located at 40°33′18″N 81°55′5″W / 40.555°N 81.91806°W / 40.555; -81.91806 (40.554901, -81.917940), along Killbuck Creek. Millersburg is located 817 feet (249 m) above sea level.
According to the United States Census Bureau, the village has a total area of 2.0 square miles (5.2 km²), all of it land.