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Jacobus is a borough in York County, Pennsylvania, United States. The population was 1,841 at the 2010 census.
Jacobus is located at 39°52′57″N 76°42′43″W / 39.8825°N 76.71194°W / 39.8825; -76.71194 (39.882516, -76.712068).
According to the United States Census Bureau, the borough has a total area of 0.9 square miles (2.4 km²), all of it land.