Williston is a town in Barnwell County, South Carolina, United States. The population was 3,307 at the 2000 census.
The town lies in the center of the Charleston-Hamburg railroad line, the line of the Best Friend locomotive. The train tracks were removed in the 1990s, however, the track site has been designated as historically significant by the South Carolina Department of Archives. This section of the train route is now the route of the South Carolina Heritage Corridor.
There are 3 schools in the town: Williston-Elko High School, Williston-Elko Middle School, and Kelly Edwards Elementary School. A private school in Blackville, SC, Jefferson-Davis Academy, also serves a portion of the town's population.