Unicoi is a town in Unicoi County, Tennessee, United States. The population was 3,519 at the 2000 census. It is part of the Johnson City Metropolitan Statistical Area, which is a component of the Johnson City–Kingsport–Bristol, TN-VA Combined Statistical Area – commonly known as the "Tri-Cities" region.
Unicoi is located at 36°13′12″N 82°20′17″W / 36.22°N 82.33806°W / 36.22; -82.33806 (36.220028, -82.338138).
According to the United States Census Bureau, the town has a total area of 16.3 square miles (42.1 km²), all of it land.