Galesville is a city in Trempealeau County, Wisconsin, United States. The population was 1,481 at the 2010 census.
Galesville is located where Beaver Creek flows into a wide area of the Mississippi River valley. The creek is impounded to form Lake Marinuka.
Galesville is located at 44°5′1″N 91°21′13″W / 44.08361°N 91.35361°W / 44.08361; -91.35361 (44.083690, -91.353576).