Trempealeau is a village in Trempealeau County in the U.S. state of Wisconsin. The population was 1,319 at the 2000 census. The village is located within the Town of Trempealeau.
Trempealeau is located at 44°0′24″N 91°26′4″W / 44.00667°N 91.43444°W / 44.00667; -91.43444 (44.006906, -91.434572).
According to the United States Census Bureau, the village has a total area of 1.3 square miles (3.3 km²), of which, 1.2 square miles (3.1 km²) of it is land and 0.1 square miles (0.2 km²) of it (6.25%) is water.